27-appendix-g.Rmd 1020 Bytes
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---
output:
  pdf_document: default
  html_document: default
---
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# Sequencing of the DNA sequence used for the expression of of the $\alpha7$ ECD-2GSC

\newpage

(ref:appf) **Sequencing of $\alpha7$ ECD-2GSC cloned into pET27-pelB-3C expression vector.** Inserted into pET27-pelB-3C vector $\alpha7$ ECD-2GSC was sequenced using universal T7 Terminator reverse primer and primer within the 3' region of MBP (a). The cloned sequence (Query) was compared to the expected sequence (Subject) (b). The sequence was translated and merged with the pel-3C to depict the entire protein expressed (c). The major domains are highlighted, as well as the mutated Cys-loop (in red). 

```{r echo=FALSE}
knitr::include_graphics("fig/results5/png/alpha7-2gsc-seq1.png")
```

```{r echo=FALSE}
knitr::include_graphics("fig/results5/png/alpha7-2gsc-seq2.png")
```

```{r appe-label, fig.cap = "(ref:appf)", fig.scap = "Sequence of the $\\alpha7$ ECD-2GSC", echo=FALSE}
knitr::include_graphics("fig/results5/png/alpha7-2gsc-seq3.png")
```