22-appendix-b.Rmd 1.14 KB
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# DNA sequence used for the expression of *eat-2* in the pharyngeal muscle of *C. elegans*. 
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\newpage

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(ref:app2) Sequencing of *myo-2-eat-2* from the *pDEST* vector. Myo-2::eat-2 nucleotide fragment from the expression vector used to generate *C. elegans* transgenic strains was sequenced following cloning. 3 forward (Fw) and a reverse (Rev) primer were used to generate overlaping sequencing fragments spaning the entire sequence of interets (a). Sequencing results authenticated the identity of the construct (b) and confirmed the amino acid sequence of the *eat-2* gene.
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```{r echo=FALSE, out.height = '80%'}
knitr::include_graphics("fig/results4/PNG/1-myo2-eat-2.png")
```

```{r echo=FALSE}
knitr::include_graphics("fig/results4/PNG/2-myo2-eat2.png")
```

```{r echo=FALSE}
knitr::include_graphics("fig/results4/PNG/3-myo2-eat2.png")
```

```{r echo=FALSE}
knitr::include_graphics("fig/results4/PNG/4-myo2-eat2.png")
```

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\newpage
```{r app2-label, fig.cap="(ref:app2)", fig.scap= "Sequencing of \\textit{myo-2-eat-2} from the \\textit{pDEST} vector", include="TRUE", results="show", echo=FALSE}
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knitr::include_graphics("fig/results4/PNG/5-myo2-eat2.png")
```