27-appendix-g.Rmd 1.09 KB
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---
output:
  pdf_document: default
  html_document: default
---
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# Sequencing of the DNA sequence used for the expression of of the $\alpha7$ chimera protein
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\newpage

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(ref:appf) **Sequencing of *$\alpha7$ ECD-2GSC* cloned into *pET27-pelB-3C* expression vector.** Inserted into *pET27-pelB-3C* vector *$\alpha7$ ECD-2GSC* was sequenced using universal T7 Terminator reverse primer and primer within the 3' region of MBP (a). The cloned sequence (Query) was compared to the expected sequence (Subject) (b). The sequence was translated and merged with the pel-3C to depict the entire protein expressed (c). The major domains are highlighted, as well as the mutated Cys-loop (in red). 
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```{r echo=FALSE}
knitr::include_graphics("fig/results5/png/alpha7-2gsc-seq1.png")
```

```{r echo=FALSE}
knitr::include_graphics("fig/results5/png/alpha7-2gsc-seq2.png")
```

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```{r appe-label, fig.cap = "(ref:appf)", fig.scap = "Sequence of the $\\alpha7$ \\textit{ECD-2GSC} cloned into \\textit{pET27-pelB-3C} expression vector.", fig.align='center', echo=FALSE}
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knitr::include_graphics("fig/results5/png/alpha7-2gsc-seq3.png")
```