26-appendix-f.Rmd 3.27 KB
 mk11g11 committed Feb 19, 2020 1 # DNA sequence used for the expression of human $\alpha7$ extracellular domain in *E. coli*  mk11g11 committed Sep 29, 2019 2   mk11g11 committed Apr 13, 2020 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24   mk11g11 committed Feb 19, 2020 25   mk11g11 committed Apr 13, 2020 26 (ref:appe) **Sequencing of *pelB-3C* cloned into *pET27* expression vector.** Inserted into *pET27 pelB-3C* sequence was sequenced using universal T7 forward and T7 terminator primers (a). The cloned sequence (Query) was compared to the expected sequence (Subject) (b). Single nucleotide mutation from A to C occured, highlighted in red, changing the codon from GCC to GCA, both of which encode for alanine. The cloned nucleotide sequence was translated (c) and major functional domains, as well highlighted.  mk11g11 committed Feb 19, 2020 27   mk11g11 committed Apr 13, 2020 28 \newpage  mk11g11 committed Feb 19, 2020 29   mk11g11 committed Apr 13, 2020 30 31 32 {r out.height = '90%', fig.align='center', echo=FALSE} knitr::include_graphics("fig/results5/png/pelb-3c_seq_1.png")   mk11g11 committed Feb 19, 2020 33   mk11g11 committed Apr 13, 2020 34 35 36 {r fig.align='center', echo=FALSE} knitr::include_graphics("fig/results5/png/pelb-3c_seq_2.png")   mk11g11 committed Feb 19, 2020 37   mk11g11 committed Apr 13, 2020 38 39 40 {r pelb-3c-lbl, fig.cap = "(ref:appe)", fig.scap= "Sequencing of \\textit{pelB-3C} cloned into \\textit{pET27} expression vector", fig.align='center', echo=FALSE} knitr::include_graphics("fig/results5/png/pelb-3c_seq_3.png")